<p>Aboveground biomass (AGB) estimates at the plot level plays a major part in connecting accurate single-tree AGB measurements to relatively difficult regional-scale AGB estimates. However, complex and spatially heterogeneous landscapes, where multiple environmental covariates (such as longitude, latitude, and forest structure) affect the spatial distribution of AGB, make upscaling of plot-level models more challenging. To address this challenge, this study proposes an approach that combines machine learning with spatial statistics to construct a more accurate plot-level AGB model. The study was conducted in a <i>Eucalyptus</i> plantation in Nanjing, China. We developed, evaluated, and compared the accuracy and performance of three different machine learning models [support vector machine (SVM), random forest (RF), and the radial basis function artificial neural network (RBF-ANN)], one spatial statistics model (P-BSHADE), and three combinations thereof (SVM & P-BSHADE, RF & P-BSHADE, RBF-ANN & P-BSHADE) for forest AGB estimates based on AGB data from 30 sample plots and their corresponding environmental covariates. The results show that the performance indices RMSE, nRMSE, MAE, and MRE of all combined models are substantially smaller than those of any individual models, with the RF & P-BSHADE combined method giving the smallest value. These results demonstrate clearly that combined models, especially the RF & P-BSHADE model, can improve the accuracy of plot-level AGB models and reduce uncertainty on plot-level AGB estimates or even on large-forested-landscape AGB estimates. These research results are important because they reduce the uncertainty in estimates of the regional carbon balance.</p>