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Biogeosciences An interactive open-access journal of the European Geosciences Union
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https://doi.org/10.5194/bg-2019-444
© Author(s) 2019. This work is distributed under
the Creative Commons Attribution 4.0 License.
https://doi.org/10.5194/bg-2019-444
© Author(s) 2019. This work is distributed under
the Creative Commons Attribution 4.0 License.

Submitted as: research article 16 Dec 2019

Submitted as: research article | 16 Dec 2019

Review status
This discussion paper is a preprint. It is a manuscript under review for the journal Biogeosciences (BG).

Calcite and vaterite biosynthesis by nitrate dissimilating bacteria in carbonatogenesis process under aerobic and anaerobic conditions

Marwa Eltarahony, Sahar Zaki, Ayman Kamal, and Desouky Abd-El-Haleem Marwa Eltarahony et al.
  • Environmental Biotechnology Department, Genetic Engineering and Biotechnology Research Institute (GEBRI), City of Scientific Research and Technological Applications (SRTA-City), 21934, New Borg El-Arab City, Alexandria, Egypt

Abstract. This study deals with 16S rDNA identified bacteria, Lysinibacillus sphaericus (71A), Raoultella planticola (VIP), and Streptomyces pluricolorescens (EM4) capable of precipitating CaCO3 through a nitrate reduction aerobically and anaerobically. The produced CaCO3 crystals were analyzed using XRD, EDX, and SEM. The results showed that the carbonatogenic bacteria served as nucleation sites for CaCO3 precipitation with distinct variation in polymorph and morphology; reflecting strain-specific property. Notably, the amount of precipitated CaCO3 recorded 3.27 (aerobic), 1.55 (anaerobic), 4.15 (aerobic), 3.75 (aerobic) and 1.87 (anaerobic) g/100 mL of strains 71A, EM4 and VIP, respectively, for 240 h of incubation. The study of changes in media chemistry during carbonatogenesis process revealed positive correlation between bacterial growth, nitrate reductase activity, pH, EC, amount of deposited CaCO3 and NO3 consumption. Therefore, the applications of these bacterial strains, which employed for the first time in carbonatogenesis process, are promising in the environmental, biomedical and civil engineering fields.

Marwa Eltarahony et al.
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Marwa Eltarahony et al.
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Short summary
This study utilized the bacterial strains in carbonatogenesis process through nitrate reduction under aerobic and anaerobic conditions. The precipitated CaCO3 were characterized, it was either calcite or vaterite. Overall, carbonatogenesis process via nitrate reduction is totally biological, ecofriendly, inexpensive, and promotes CaCO3 precipitations without accumulation of toxic by-product. The produced biosafe CaCO3 would be employed for subsequent environmental and medical applications.
This study utilized the bacterial strains in carbonatogenesis process through nitrate reduction...
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