Journal cover Journal topic
Biogeosciences An interactive open-access journal of the European Geosciences Union
Journal topic
Discussion papers
https://doi.org/10.5194/bg-2018-529
© Author(s) 2019. This work is distributed under
the Creative Commons Attribution 4.0 License.
https://doi.org/10.5194/bg-2018-529
© Author(s) 2019. This work is distributed under
the Creative Commons Attribution 4.0 License.

Submitted as: research article 12 Feb 2019

Submitted as: research article | 12 Feb 2019

Review status
This discussion paper is a preprint. It has been under review for the journal Biogeosciences (BG). The revised manuscript was not accepted.

Diversity, distribution and nitrogen use strategies of bacteria in the South China Sea basin

Yuan-Yuan Li1, Xiao-Huang Chen1, Peng-Fei Wu1, Dong-Xu Li1, Lin Lin1, and Da-Zhi Wang1,2 Yuan-Yuan Li et al.
  • 1State Key Laboratory of Marine Environmental Science/College of the Environment and Ecology, Xiamen University, Xiamen, 361005, China
  • 2Key Laboratory of Marine Ecology & Environmental Sciences, China Academy of Sciences, Qingdao, 266071, China

Abstract. The diversity and abundance of bacteria and diazotrophs in the euphotic and aphotic layers of the South China Sea (SCS) basin were investigated based on high-throughput sequencing of the 16S rRNA and nifH genes. Bacterial communities in the aphotic layers significantly differed from those at the euphotic layers, and were characterized by geographical specificities. Prochlorococcus and Alphaproteobacteria were abundant in the surface layer, whereas Gammaproteobacteria was more common in the aphotic layers. Moraxellaceae was the most abundant group in the aphotic layer in the northern basin of the SCS (nSCS), while SAR324, SAR202 and SAR406 occurred mainly in the southern basin of the SCS (sSCS). Diazotrophic Alphaproteobacteria was the predominant group in the SCS basin, whereas Marine Group II Euryarchaeota emerged in the euphotic bottom of both nSCS and sSCS. Abundances of genes encoding amino acid transporters and ammonium assimilating enzymes were relatively high in the SCS surface and the entire water column of the sSCS, while expression levels of urea and ammonium transporter-encoding genes were the highest at the surface of the SEATS site. Iron deficiency-induced gene IdiA and urease were highly expressed at the A2 site. Our results indicated that bacterial communities in the SCS were depth-stratified and exhibited geographic divergency in the aphotic layers between nSCS and sSCS. Amino acids and ammonium were the major nitrogen sources for bacteria while urea, ammonia and nitrite played important roles in regulating cell growth of Prochlorococcus in different regions of the SCS.

Yuan-Yuan Li et al.
Interactive discussion
Status: closed
Status: closed
AC: Author comment | RC: Referee comment | SC: Short comment | EC: Editor comment
Printer-friendly Version - Printer-friendly version Supplement - Supplement
Interactive discussion
Status: closed
Status: closed
AC: Author comment | RC: Referee comment | SC: Short comment | EC: Editor comment
Printer-friendly Version - Printer-friendly version Supplement - Supplement
Yuan-Yuan Li et al.
Yuan-Yuan Li et al.
Viewed  
Total article views: 266 (including HTML, PDF, and XML)
HTML PDF XML Total Supplement BibTeX EndNote
199 66 1 266 19 1 0
  • HTML: 199
  • PDF: 66
  • XML: 1
  • Total: 266
  • Supplement: 19
  • BibTeX: 1
  • EndNote: 0
Views and downloads (calculated since 12 Feb 2019)
Cumulative views and downloads (calculated since 12 Feb 2019)
Viewed (geographical distribution)  
Total article views: 231 (including HTML, PDF, and XML) Thereof 228 with geography defined and 3 with unknown origin.
Country # Views %
  • 1
1
 
 
 
 
Cited  
Saved  
No saved metrics found.
Discussed  
No discussed metrics found.
Latest update: 20 Sep 2019
Publications Copernicus
Download
Short summary
Systematic studies on diversities and nitrogen use strategies of bacterial communities in the South China Sea basin are still scarce. Here, we applied high-throughput sequencing, bioinformatic analysis and qPCR assays, and found that the depth was a significant explanatory variable for the bacterial populations from different water masses. Amino acids and ammonium were the major nitrogen sources for bacteria while urea, ammonia and nitrite played vital roles in different regions of the basin.
Systematic studies on diversities and nitrogen use strategies of bacterial communities in the...
Citation