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https://doi.org/10.5194/bg-2018-470
© Author(s) 2019. This work is distributed under
the Creative Commons Attribution 4.0 License.
https://doi.org/10.5194/bg-2018-470
© Author(s) 2019. This work is distributed under
the Creative Commons Attribution 4.0 License.

Research article 26 Feb 2019

Research article | 26 Feb 2019

Review status
This discussion paper is a preprint. A revision of the manuscript is under review for the journal Biogeosciences (BG).

Identify the core bacterial microbiome of hydrocarbon degradation and a shift of dominant methanogenesis pathways in oil and aqueous phases of petroleum reservoirs with different temperatures from China

Zhichao Zhou1, Bo Liang2, Li-Ying Wang2, Jin-Feng Liu2, Bo-Zhong Mu2, Hojae Shim3, and Ji-Dong Gu1 Zhichao Zhou et al.
  • 1Laboratory of Environmental Microbiology and Toxicology, School of Biological Sciences, The University of Hong Kong, Pokfulam Road, Hong Kong SAR, Hong Kong, People's Republic of China
  • 2State Key Laboratory of Bioreactor Engineering and Institute of Applied Chemistry, East China University of Science and Technology, Shanghai 200237, People's Republic of China
  • 3Faculty of Science and Technology, University of Macau, Macau, People's Republic of China

Abstract. Microorganisms in petroleum reservoirs mediate hydrocarbon degradation coupling with methanogenesis process as the terminal step, they also contribute to microbial enhanced oil recovery (MEOR) worldwide with great economic and environmental benefits. Here, a molecular investigation, using the 16S rRNA and mcrA gene profiles based on MiSeq sequencing and clone library construction method, was conducted on oil and water (aqueous) phases of samples with high, moderate and low temperatures from seven petroleum reservoirs in China. A core bacterial microbiome with a small proportion of shared OUT, but a high proportion of sequences among all reservoirs was discovered, including aerobic degraders, sulfate/nitrate reducing bacteria, fermentative bacteria and sulfur cycling bacteria distributed mainly in Proteobacteria, Bacteroidetes, Deferribacteres, Deinococcus-Thermus, Firmicutes, Spirochaetae and Thermotogae. Their prevalence in the reported petroleum reservoirs and successive enrichment cultures suggest their common roles and functions involved in aliphatic and aromatic hydrocarbon degradation. Dominant methanogenesis process generally shifts from hydrogenotrophic pathway in water phase to acetoclastic pathway in oil phase in high temperature reservoirs, but the opposite is true for low temperature reservoirs. No differences were detected between two phases in moderate temperature reservoirs. Physiochemical factors, including pH, temperature, phase conditions, and nitrate, Mn2+, and Mg2+ concentrations are the main ones correlating to the microbial compositional and functional profiles significantly. LEfSe analysis shows distribution differences of microbial groups towards pH, temperature, and oil/water. Tax4Fun functional profiling indicates major functional metabolism differences between the two phases, including amino acids, hydrocarbons in the oil phase, and carbohydrates in the water phase.

Zhichao Zhou et al.
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Zhichao Zhou et al.
Zhichao Zhou et al.
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Short summary
This study shows a core bacterial microbiome with a small proportion of shared OUT of common sequences among all oil reservoirs. Dominant methanogenesis shifts from hydrogenotrophic pathway in water phase to acetoclastic pathway in the oil phase at high temperature, but the opposite is true at low temperature. There are also major functional metabolism differences between the two phases for amino acids, hydrocarbons and carbohydrates.
This study shows a core bacterial microbiome with a small proportion of shared OUT of common...
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