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© Author(s) 2018. This work is distributed under
the Creative Commons Attribution 4.0 License.
© Author(s) 2018. This work is distributed under
the Creative Commons Attribution 4.0 License.

Submitted as: research article 19 Nov 2018

Submitted as: research article | 19 Nov 2018

Review status
This discussion paper is a preprint. A revision of the manuscript is under review for the journal Biogeosciences (BG).

Particulate trace metal dynamics in response to increased CO2 and iron availability in a coastal mesocosm experiment

M. Rosario Lorenzo1, María Segovia1, Jay T. Cullen2, and María T. Maldonado3 M. Rosario Lorenzo et al.
  • 1Department of Ecology, Faculty of Sciences, University of Málaga, Bulevar Louis Pasteur s/n, 29071-Málaga, Spain
  • 2School of Earth and Ocean Sciences, University of Victoria, 3800 Finnerty Road, Bob Wright Centre A405, Victoria BC V8P 5C2, Canada
  • 3Department of Earth and Ocean and Atmospheric Sciences, University of British Columbia, 2207 Main Mall, Vancouver BC V6T 1Z4, Canada

Abstract. Rising concentrations of atmospheric carbon dioxide are causing ocean acidification and will influence marine processes and trace metal biogeochemistry. The importance of the combined impacts of elevated CO2 and changes in trace metal availability on marine plankton remain largely unknown. A mesocosm experiment was performed to study changes in particulate trace metal concentrations during a bloom dominated by the coccolithophore Emiliania huxleyi. We employed a full-factorial experimental design, comprising all combinations of ambient and elevated pCO2 and dissolved iron (dFe). Particulate metal concentrations (Fe, Cu, Zn, Co, Mn, Cd, Mo, Ti and Pb) were determined by high-resolution inductively coupled plasma mass spectrometry (HR-ICPMS). We examined biogenic and lithogenic sources of particulate metals, and their evolution during the experiment. Biogenic metal concentrations were estimated from bulk particle measurements by comparing phosphorus (P)-normalised quotas with published ratios, as well as concentrations of particulate trace metals in the presence and absence of an oxalate-EDTA wash. Our results demonstrate that particulate Ti and Fe concentrations were dominated by lithogenic material in the fjord. In contrast, particulate Cu, Co, Mn, Zn, Mo and Cd concentrations correlated with P concentrations and phytoplankton biomass, indicative of their strong biogenic character. Furthermore, ocean acidification changed the relative concentrations of particulate metals; a result mainly driven by the effects of ocean acidification on the growth of different phytoplankton phyla. This study demonstrates the utility and robustness of combining trace metal analyses of particles in a controlled mesocosm experiment with manipulations of CO2 and Fe concentrations using natural assemblages of marine phytoplankton.

M. Rosario Lorenzo et al.
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Status: final response (author comments only)
Status: final response (author comments only)
AC: Author comment | RC: Referee comment | SC: Short comment | EC: Editor comment
M. Rosario Lorenzo et al.
M. Rosario Lorenzo et al.
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