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Biogeosciences An interactive open-access journal of the European Geosciences Union
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Discussion papers
https://doi.org/10.5194/bg-2016-202
© Author(s) 2016. This work is distributed under
the Creative Commons Attribution 3.0 License.
https://doi.org/10.5194/bg-2016-202
© Author(s) 2016. This work is distributed under
the Creative Commons Attribution 3.0 License.

Submitted as: research article 19 May 2016

Submitted as: research article | 19 May 2016

Review status
This discussion paper is a preprint. It has been under review for the journal Biogeosciences (BG). The revised manuscript was not accepted.

Monodeuterated methane: an isotopic probe to measure biological methane metabolism rates and track catabolic exchange reactions

Jeffrey J. Marlow1,a, Joshua A. Steele1,b, Wiebke Ziebis2, Silvan Scheller1, David Case1, and Victoria J. Orphan1 Jeffrey J. Marlow et al.
  • 1Division of Geological and Planetary Sciences, California Institute of Technology, Pasadena, CA, 91125 USA
  • 2Department of Biological Science, University of Southern California, Los Angeles, CA, 90089 USA
  • aCurrent address: Dept. of Organismic and Evolutionary Biology , Harvard University, Cambridge, MA 02138 USA
  • bCurrent address: Southern California Coastal Water Research Project, Costa Mesa, CA 92626 USA

Abstract. Biological methane oxidation is a globally relevant process that mediates the flux of an important greenhouse gas through both aerobic and anaerobic metabolic pathways. However, measuring the rates of these metabolisms presents many obstacles, from logistical barriers to regulatory hurdles and poor precision. Here we present a new approach for measuring rates of microbial methane metabolism that is non-toxic, rapid, and relatively high throughput, alleviating some of the current methodological challenges. Specifically, we tested the potential for using monodeuterated methane (CH3D) as a metabolic substrate for measuring the rate of methane activation by quantifying the change in the aqueous D / H ratio over time using a water isotope analyzer. This method represents a non-toxic, comparatively rapid and straightforward approach that is complementary to existing radio (14C)- and stable (13C) carbon isotopic methods; by probing hydrogen atom dynamics, it offers an additional dimension through which to examine the rates and pathways of methane metabolism. We provide direct comparisons between the CH3D procedure and the well-established 14CH4 radiotracer approach for several methanotrophic systems, including type I and type II aerobic methanotroph cultures, and methane seep sediment and carbonate rocks under anoxic and oxic incubation conditions. We also employ this method to investigate the role of pressure on methane oxidation rates in anoxic seep sediment, revealing an 80 % increase at the equivalent of ~ 900 m water depth (40 MPa).

The monodeuterated methane approach offers a procedurally straightforward, reliable method that advances three specific aims: 1) the direct comparison of methane oxidation rates between different experimental treatments of the same inoculum; 2) the determination of an absolute scaling factor using paired CH3D and 14C-radiocarbon procedures for new systems of interest; and 3) a continued evaluation of C- and H-atom tracking through methanotrophic metabolisms, with specific foci on enzyme reversibility and anabolic/catabolic branch points. The procedural advantages, consistency, and novel research questions enabled by the monodeuterated methane method should prove useful in a wide range of culture-based and environmental microbial systems to further elucidate methane metabolism dynamics.

Jeffrey J. Marlow et al.
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AC: Author comment | RC: Referee comment | SC: Short comment | EC: Editor comment
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Interactive discussion
Status: closed
Status: closed
AC: Author comment | RC: Referee comment | SC: Short comment | EC: Editor comment
Printer-friendly Version - Printer-friendly version Supplement - Supplement
Jeffrey J. Marlow et al.
Jeffrey J. Marlow et al.
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Short summary
Methane is a powerful greenhouse gas that plays a major role in the planet's biogeochemical cycles and climate system. Measuring rates of microbial methane consumption is an important capability that allows scientists to better quantify fluxes between reservoirs and assess potential impacts. This study presents a new method for measuring these rates using monodeuterated methane; the approach is logistically streamlined and offers a distinct isotopic dimension for methane metabolism analysis.
Methane is a powerful greenhouse gas that plays a major role in the planet's biogeochemical...
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