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Biogeosciences An interactive open-access journal of the European Geosciences Union
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https://doi.org/10.5194/bg-2016-162
© Author(s) 2016. This work is distributed under
the Creative Commons Attribution 3.0 License.
https://doi.org/10.5194/bg-2016-162
© Author(s) 2016. This work is distributed under
the Creative Commons Attribution 3.0 License.

Submitted as: research article 30 May 2016

Submitted as: research article | 30 May 2016

Review status
This discussion paper is a preprint. It has been under review for the journal Biogeosciences (BG). The revised manuscript was not accepted.

Microtopography matters for CH4 formation in a peat soil: a combined inhibitor and 13C study

Johannes Krohn1, Ivana Lozanovska1,a, Yakov Kuzyakov1,2, and Maxim Dorodnikov1 Johannes Krohn et al.
  • 1Department of Soil Science of Temperate Ecosystem s , Faculty of Forest Sciences and Forest Ecology, Georg - August University Göttingen, Bü sgenweg 2, 37077 Göttingen, Germany
  • 2Department of Agricultural Soil Science, Georg - August University Göttingen, Bü sgenweg 2, 37077 Göttingen, Germany
  • apresent adress : Macedonian Ecological Society, Arhimedova 5, 1000 Skopje, Macedonia

Abstract. Abstract. Peatlands’ microtopography units – hummocks and hollows – are mainly differing by hydrological characteristics (water table level, i.e. oxic-anoxic conditions) and vegetation communities. These factors affect the fluxes of key greenhouse gases (GHG) – methane (CH4) and carbon dioxide (CO2). However, the effects of microrelief forms on belowground CO2 and CH4 production and pathways of methanogenesis need deeper understanding. We hypothesized increasing CH4 and CO2 production potentials from naturally drier hummocks to more wet hollows during anaerobic incubation. GHG production in peat was expected to decrease with depth (decreasing inputs of recent plant-derived deposits) but the contribution of hydrogenotrophic vs. acetoclastic pathway to the total methanogenesis should be higher in deeper peat layers as compared to upper layers. To test the hypotheses, we measured CH4 and CO2 productions together with the respective δ13C values under controlled anaerobic conditions with- and without addition of specific inhibitor of methanogenesis (2-bromo-ethane sulfonate, BES) in a peat soil of hummocks and hollows of five depths (15, 50, 100, 150 and 200 cm). The concentration of BES (1 mM) aimed to block acetoclastic but not the hydrogenotrophic pathway of methanogenesis. As expected, CH4 production was ca. 2 times higher in hollows than in hummocks, though no differences in CO2 were measured between the microforms. With depth, CO2 production rates decreased by 77 % (15 cm vs. 200 cm) in both microforms, whereby the highest CH4 production was measured at 15 cm in hollows (91 % of total produced CH4) and at 50 cm in hummocks (82 %). Noteworthy, at 15 cm of hummocks less than 1 % of total CH4 production was observed. Decreasing GHG production rates with depth positively correlated to an increase in the extractable total N and NH4+ concentrations. The hydrogenotrophic pathway of methanogenesis in deep vs. surface layers was depicted by lower (more negative) δ13C-CH4 and higher δ13C-CO2 values, respectively. Between the microforms, overall higher contribution of hydrogenotrophic vs. acetoclastic methanogenesis corresponded to hollows as compared to hummocks. Contrary to the expectation, the addition of 1mM BES was not selective and inhibited both pathways of methanogenesis. Concluding, peatlands’ microrelief is an important factor regulating the GHG fluxes. However, the effects of microforms on the production of CH4 and pathways of methanogenesis were pronounced for the upper 50 cm layer. Finally, inhibition with BES appeared to be less effective tool for the partitioning between pathways of methanogenesis as compared with the isotope method.

Johannes Krohn et al.
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Interactive discussion
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Status: closed
AC: Author comment | RC: Referee comment | SC: Short comment | EC: Editor comment
Printer-friendly Version - Printer-friendly version Supplement - Supplement
Johannes Krohn et al.
Johannes Krohn et al.
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