Abstract. Several in situ iron-enrichment experiments have been conducted, where the response of the phytoplankton community differed. We use a marine ecosystem model to investigate the effect of iron on phytoplankton in response to different initial plankton conditions and mixed layer depths. Sensitivity analysis of the model results to the mixed layer depths reveals that the modeled response to the same iron enhancement treatment differed dramatically according to the different mixed layer depth. The magnitude of the iron-induced biogeochemical responses in the surface water, such as maximum chlorophyll, is inversely correlated with the mixed layer depth, as observed. The significant decrease in maximum surface chlorophyll with mixed layer depth results from the difference in diatom concentration in the mixed layer, which is determined by vertical mixing. Sensitivity of the model to initial mesozooplankton (as grazers on diatoms) biomass shows that column-integrated net community production and export production are strongly controlled by the initial mesozooplankton biomass. Higher initial mesozooplankton biomass yields high grazing pressure on diatoms, which results in less accumulation of diatom biomass. The initial diatom biomass is also important to the outcome of iron enrichment but is not as crucial as the mixed layer depth and the initial mesozooplankton biomass. This modeling study suggests not only mixed layer depth but also the initial biomass of diatoms and its principle grazers are crucial factors in the response of the phytoplankton community to the iron enrichments, and should be considered in designing future iron-enrichment experiments.